recA gene product is responsible for inhibition of deoxyribonucleic acid synthesis after ultraviolet irradiation.

نویسندگان

  • Z Trgovcević
  • D Petranović
  • M Petranović
  • E Salaj-Smic
چکیده

Deoxyribonucleic acid synthesis after ultraviolet irradiation was studied in wild-type, uvrA, recB, recA recB, and recA Escherichia coli strains. Inhibition of deoxyribonucleic acid synthesis, which occurs almost immediately after exposing the cells to ultraviolet radiation, depends on the functional gene recA.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Host cell deoxyribonucleic acid polymerase I and the support of T4 bacteriophage growth.

Ultraviolet irradiation of Escherichia coli polA(-) cells reduces their capacity to support the growth of T4 phage. There is no additional loss of capacity observed in pol tsA(-)recA(-) double mutants at the nonpermissive temperature. The reversion frequency of a T4 rII mutant after ultraviolet irradiation is not changed by the absence of host deoxyribonucleic acid polymerase I.

متن کامل

Recombination-deficient mutant of Streptococcus faecalis.

An ultraviolet radiation-sensitive derivative of Streptococcus faecalis strain JH2-2 was isolated and found to be deficient in recombination, using a plasmid-plasmid recombination system. The strain was sensitive to chemical agents which interact with deoxyribonucleic acid and also underwent deoxyribonucleic acid degradation after ultraviolet irradiation. Thus, the mutant has properties similar...

متن کامل

Nucleic acid synthesis in ultraviolet-irradiated Bacillus cereus.

Kelner (J. Bacteriol., 65, 252, 1953), has found that ultraviolet irradiation inhibits deoxyribonucleic acid synthesis in Escherichia coli strain B/r. He sometimes observed a resumption of this synthesis after a certain period of postirradiation incubation. This has also been reported by Kanazir and Errera (Cold Spring Harbor Symposia Quant. Biol., 21, 19, 1956). As most of the irradiated cells...

متن کامل

Recovery from ultraviolet light-induced inhibition of DNA synthesis requires umuDC gene products in recA718 mutant strains but not in recA+ strains of Escherichia coli.

Ultraviolet light (UV) inhibits DNA replication in Eschericia coli and induces the SOS response, a set of survival-enhancing phenotypes due to derepression of DNA damage-inducible genes, including recA and umuDC. Recovery of DNA synthesis after UV irradiation ("induced replisome reactivation," or IRR) is an SOS function requiring RecA protein and postirradiation synthesis of additional protein(...

متن کامل

Ultraviolet irradiation inhibition of replicative deoxyribonucleic acid synthesis in toluene-treated Escherichia coli.

Toluene-treated cells of Escherichia coli represent an in uifro system capable of replicative DNA synthesis. They should offer advantages for investigation of enzymatic mechanisms of DNA repair synthesis secondary to ultraviolet irradiation. The stability of this system allows in vifro irradiation with subsequent measurement of DNA synthesis. Ultraviolet irradiation in vitro causes an inhibitio...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 143 3  شماره 

صفحات  -

تاریخ انتشار 1980